If it is much lower, then the salts should be increased to bridge the negative charges.Tags: Hypnotherapy Business PlanEssay Tentang Lingkungan HidupEdwin Morgan Glasgow Sonnet 1 EssayGreat Gatsby Thesis PaperHow To Build Critical Thinking SkillsEnglishdaily626 Lower Secondary English EssaysState Of Arkansas Commission On Law Enforcement Standards And Training Personal History StatementCruel Angels Thesis MusicEssay Typer Not WorkingComputer Science Thesis Vs Project
Up until the point of writing your methodology, you will have defined your research question and conducted a detailed review of what other scholars in the field have to say about your topic.
You’ll have also reviewed the in which these scholars have arrived at their conclusions – the assumptions on which their work is based, the theoretical frameworks they've used, and the methods they've used to gather, marshal and present their data.
You will have used these observations, along with discussions with your supervisor, to plan how you're going to tackle your research question.
This could be planning how you'll gather data, or what models you'll use to process it, or what philosophical positions most inform your work.
Following this, your dissertation methodology provides a detailed account of both Your methodology needs to establish a clear relationship between your research question, the existing scholarship in your field that you have surveyed as part of your literature review, and the means by which you'll come to your conclusions.
Therefore, no matter what subject area you're working in, your methodology section will include the following: While the outline of your methodology section will look much the same regardless of your discipline, the details are liable to be quite different depending on the subject area in which you're studying.
The best advice is to read the methods section of papers similar to your paper.
Here are a few examples of aptamer papers with well written methods section: Stoltenburg, R, Reinemann, C, and Strehlitz, B: Flu Mag-SELEXas an advantageous method for DNA aptamer selection. Wilner, SE, Wengerter, Br, Maier, K, de Lourdes Borba Magalhaes, M, Del Amo, DS, Pai, S, Opazo, F, Rizzoli, SO, Yan, A, Levy, M: An RNA Alternative To Human Transferrin: ANew Tool for Targeting Human Cells.
All of this information is useful for suggesting buffer conditions.
Ideally, an isoelectric point above 7 will be positively charged at neutral p H and therefore more likely that the nucleic acid will bind.